AZ-800M三维立体及样带植物荧光成像系统

AZ-800M系列叶绿素荧光成像系统用于检测植物发出荧光的动态变化和空间分布，Kautsky效应过程、荧光淬灭及其它瞬时荧光过程（瞬变）都可被摄取，从而提供2维荧光图像，计算常规的荧光参数如F0， FM， FV， F0’， FM’， FV’， NPQ， ΦPSII， FV/FM， FV’/FM’， RFd， qN， qP，这些荧光参数图像可用于研究植物的光合效率及果实的成熟过程，还可研究因病变、衰老、环境胁迫或突变造成的荧光变化。测量样品范围广，既可是单个叶绿体或单个细胞（用显微荧光成像系统），也可是小的冠层（用拱形植物荧光成像系统）或样带区域（区域植物荧光成像系统）。此外，荧光蛋白成像系统可以成像各种荧光蛋白发射荧光过程。

特点：

三维立体植物荧光成像系统和样带植物荧光成像系统，是根据客户定制的大型活体植物荧光成像系统，是目前世界上唯一可在野外成像区域植物荧光的系统。分样带植物荧光成像系统和拱形（三维立体）植物荧光成像系统等。
1. 样带植物荧光成像系统：分单向和双向（XY）两种，根据客户需求可以有不同规格。单向荧光成像系统可对长达2.5米的样带进行荧光成像测量，双向荧光成像的样带可达1x1.8或1米x2米。本系统用于对样带内的植物进行扫描荧光成像，还可用于监测植物对不同梯度胁迫因子的响应。
2. 拱形（三维立体）植物荧光成像系统：可以对植物进行不同角度的扫描成像，并通过相应软件形成植物的冠层三维荧光图像。

Open FluorCam FC 800-O/1010

Open FluorCam is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. The position of the camera may also be adjusted for added precision. The maximum area for imaging with standard hardware is 13 x 13 cm, respectively 20 x 20 cm, depending on the size of selected light sources. Much larger areas, up to 200 x 100 cm, can be imaged by the addition of LED panels and/or by mounting the whole system on a movable frame - see the XY-Plane, Transect, Arch, and Rover Customized FluorCams, or Open FluorCam video on this page.

Applications:

• Screening for photosynthetic performance.
• Stress resistance or susceptibility.
• Stomatal patchiness.
• Metabolic perturbations.
• Growth and yield.

Samples:

• Leaves, plants, fruits, vegetables.
• Cyanobacteria, green algae.
• Sample size up to 13 cm x 13 cm, respectively 20 x 20 cm.
• Imaging masks for 384-well plate, 96-well plate, Petri dish, etc.
• Dark room for adaptation as an option.

Wheat Leaf Image

Experiments and Measured Parameters:

• Quenching.
• Kautsky induction.
• QA reoxidation (needs optional accessories).
• OJIP (needs optional accessories).
• Fast fluorescence induction with 1µs resolution (needs optional accessories).
• PAR absorptivity (needs optional accessories).
• Measured parameters: Fo, FM, FV, Fo’, FM’, FV’, QY(II).
• More than 50 calculated parameters: NPQ, FV/FM, FV’/FM’, Rfd, qN, qP, PAR-absorptivity, electron transport rate (ETR), and many others.

Different Emission Bands:

• 8 position filter wheel (optional).
• Chlorophyll fluorescence (high pass 695 nm, low pass 780 nm), GFP (high pass 495, low pass 660 nm, band pass 505/560 nm), PAR (clear glass), YFP, CY3, CY5, as well as other fluorescence colors.

Images of More Than 50 Calculated Parameters in One Measurement

Light Sources:

• Actinic light up to 3,000 µmol(photons)/m².s.
• Super pulse up to 7,500 µmol(photons)/m².s.
• STF - Single Turnover Flash.
• IR 735 nm (FAR).
• The FluorCam system includes four super bright LED panels.
• Two panels provide Measuring Light and Actinic Light 1 (red light 618 nm in a standard version). Two panels provide Actinic Light 2 and Saturating Pulse ( these two panels are mounted according to customer's needs in blue (455 nm), red (618 nm), or white color).
• Other wavelengths are available as option: 390 nm, 470 nm, 505 nm, 570 nm, 605 nm, 630 nm, 735 nm, and others.
• Additional light sources, mounted close to the camera stand or to the device side, may be provided, e.g., infra-red, ultra-violet, or green (optional).
• Variable excitation color.

Standard Imaging Format:

• 512 x 512 pixels.
• A/D: 12 bit (4096 grey levels).
• 8.2 µm x 8.4 µm pixel size.
• 50 images per second.
• Advantageous for rapid processes measurements.

Optional Imaging Format:

• 640 x 480 pixels and 1392 x 1040 pixels, respectively.
• A/D: 12 bit (4096 grey levels).
• 6.45 µm x 6.45 µm pixel size.
• 30 and 15 images per second, respectively.
• Great for slow processes measurements and for applications where the higher spatial resolution is of high importance.

FluorCam Software Window

NEW FluorCam 7.0 Software

• Automated experimental protocols via a Windows Wizard.
• Multiple (automatically repeated) experiments.
• Automated labeling of individual plants, or samples, within the field of view.
• Kinetic analysis of data from all samples within the field of view.
• Numerous image manipulation tools.
• Barcode reader support.
• Export to Excel.
• Windows 2000, XP, Vista compatible*.

* Windows 2000, Windows XP and Vista are trademarks of Microsoft Corporation.

Dark Adaptation Box