FC 1000-H/GFP手持式荧光蛋白成像系统

便携式荧光蛋白成像系统是便携式植物荧光成像系统的改型版，用于植物、动物或其组织器官及菌落等绿色荧光蛋白（GFP）分布异质性成像分析研究，由四组高亮度发光二极管提供高强度测量光或适度可持续光，高强度测量光脉冲可以使荧光信号成像测量在很高的背景光下进行。荧光信号通过高灵敏度CCD摄像头探测成像，动态荧光图象可以通过所附带的软件进行分析。测量目标可以是小型植物、小型动物、绿藻、细菌菌落等活体生物荧光成像，还可进行凝胶荧光成像。根据客户研究需求，还可以通过不同的滤波器和发光二极管组合进行其它颜色荧光的成像测量，EBFP、ECFP、EYFP、DsRed等。

技术指标：

1. 测量单元由高端CCD摄像头、目镜和样品室组成，测量面积可达3.5x3.5cm；
2. CCD摄像头象素为512x512，具F1/1.4变焦目镜，每秒可拍摄50张图片，图象经由USB2.0传输到计算机；
3. 测量光闪或持续光由4个发光二极管板提供，每个光源板由25个蓝色LEDs（λmax = 488 nm）组成，光闪时间10μs——250μs可调，光强可达2000 μmol photons m-2 s-1，样品范围内光源均匀度在5％之内（面积3.5 X 3.5cm）；
4. 软件可以设置、调节实验设计如时间光源强度等、即时显示选定区域样品荧光动态变化图象、通过减掉背景光或通过平均法提高信噪比等。

Handy GFPCam FC 1000-H/GFP

Handy GFPCam is a modified version of the HandyCam. It is primarily used for imaging of fluorescence protein emission in samples with dimensions ranging from millimeters to centimeters.
Handy GFPCam is typically produced with 490 nm excitation and 510 nm emission filters and with super-bright blue LEDs (455 nm). This combination is best suited for imaging of the popular EGFP (red-shifted) variant.
The device is easily portable. It is supplied with a battery recharger and a small power supply for lab experiments. Both the batteries and the power supply fit into provided convenient bag.

Features:

• Both lab and outdoor applications.
• Measuring unit size only 21.5 cm x 13.5 cm x 13.5 cm.
• Running on batteries or power supply.
• Easily portable in a convenient bag.
• Ultra-high sensitivity CCD camera.
• Coming with a high-end computer and user-friendly software.

Applications:

• Agriculture and orticulture.
• Molecular and cellular biology.
• Phytopathology.
• Screening for genotype resistance.

Typical Experimental Organisms :

• Small plants, plant segments, small animals.
• Cyanobacteria (blue-green algae), green algae.
• Cyanobacterial or algal colonies.
• Sample size up to 3.5 cm x 3.5 cm.

Arabidopsis Image Captured by the Handy GFPCam:
- GFP Mutant Expresses in Colors
- Wildtype Expresses in Gray (Bottom)

Light Sources:

• Four light panels, each containing 25 LEDs (455 nm).
• Panel size: 40 x 40 mm.
• Standard configuration: blue light 455 nm (actinic light, superpulse and measuring flashes.
• Measuring flash duration 10 µs - 250 µs.
• Continuous actinic light adjustable in duration and power (up to 2,000 µmol(photons)/m².s).

Imaging:

• CCD format: 512 x 512; A/D: 12 bit; 50 images per second.
• F1/1.4 objective with zoom.

NEW FluorCam 7.0 Software

• Automated experimental protocols via a Windows Wizard.
• Multiple (automatically repeated) experiments.
• Intelligent image segmentation.
• Automated labeling of individual plants, or samples, within the field of view.
• Kinetic analysis of data from all samples within the field of view.
• Numerous image manipulation tools.
• Subtracting backround signals and image averaging.
• Export to Excel.
• Windows 2000, XP, Vista compatible.

Corn Kernels Captured by the Handy GFPCam:
- GFP Variant in the Upper Lines
- Control Kernels in the Bottom Lines